The type locality is a second order stream, known as an igarapé, and surrounded by secondary forest in regeneration. The bottom of is mainly composed of silt, with litter on the margins and few branches and trunks of a diameter greater than 10 cm. In the pools, there is flocculant sediment that is easily disturbed from submerged litter and macrophytes were found at sites with a little more water flow. Most individuals were only observed in more lentic environments, swimming in small schools of 5 to 20 individuals. Individuals also observed to swim alone and together with other species of Characidae, such as Moenkhausia phaeonota and Hyphessobrycon heliacus near igarapé margins with depth ranging from 20-40 cm. Individuals were also sometimes observed foraging among the submerged litter in the igarapé margins. This species also inhabits faster water, no vegetation and substrata consisting of sand and mud .
This species is distinguished from all its congeners by having a black, oblique stripe that largely parallels and covers the second and third anal-fin rays, extending from the origin of the second branched ray to the distal end of the third branched anal-fin ray (vs. anal fin hyaline, with uniformly distributed chromatophores, or margin blackish in all other congeners); further distinguished from other congeners by the absence of a conspicuous black midlateral stripe on the body and inner premaxillary teeth with up to seven cusps; differs from Hemigrammus unilineatus (Gill 1858), a non-congener but similar species, by having A 20-23 (vs. 24-25), maxilla with 2 or 3 tetra to heptacuspidad maxillary teeth (vs. 3-7 tricuspidad maxillary teeth) and caudal fin without scales (vs. caudal-fin lobes scaled) .Cross section: compressed.
Behavioural data for Hyphessobrycon Pinnistriatus is currently being reviewed.
Diet information for Hyphessobrycon Pinnistriatus is currently being compiled.
Reproduction data for Hyphessobrycon Pinnistriatus is currently being compiled.
Freshwater; pelagic. Tropical South America: Brazil (Mato Grosso State).
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