The species is common on coral reefs, about 2-10 m and is generally commensal with large sea anemones, either Entacmaea quadricolor (Rüppell & Leuckart, 1828) or Heteractis crispa (Ehrenberg, 1834). It is usually seen in groups that swim a short distance above their host anemones, apparently feeding on zooplankton. Reported to have been frequently encountered on local reefs in about 6-8 m depth, usually in association with Entacmaea quadricolor (Paul Brown, National Park of American Samoa, pers.comm.) . Oviparous, distinct pairing during breeding . Eggs are demersal and adhere to the substrate . Males guard and aerate the eggs .
Dorsal spines (total): 10; Dorsal soft rays (total): 16 - 18; Anal spines: 2; Anal soft rays: 14. This pomacentrid species is distinguished by the following characters: D X, 16-18 (usually X, 17); A II, 14; pectoral rays 18 (rarely 17); tubed lateral-line scales 36-43; gill rakers 5 + 12-14 (total 17-19); opercular spinules 11-19; body depth 1.7-1.9 in SL; body color generally red-orange including fins, grading to brownish on upper back of adults and a single white bar immediately posterior to the eye, its greatest width equal to that of eye or greater, narrowing to one-third to one-half of greatest width at dorsal midline .
Behavioural data for Amphiprion Barberi is currently being reviewed.
Oviparous, distinct pairing during breeding . Eggs are demersal and adhere to the substrate . Males guard and aerate the eggs .
Marine; reef-associated; depth range 2 - 10 m . Tropical Central Pacific: Fiji, Tonga and American Samoa.
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