Inhabits permanent and temporary waters, preferring clear water without much aquatic vegetation and frequenting bottoms of small stones or course sand. Hides in the substrate, often staying for considerable periods of time. Largest adults were taken from small puddles in rice fields that had dried up after irrigation was suspended. Associates with Gambusia punctata, G. puncticulata and Cichlasoma tetracanthus . Not a seasonal killifish .
Physical description for Isle of Pines rivulus is currently being compiled.
Behavioural data for Isle of Pines rivulus is currently being reviewed.
Freshwater; benthopelagic; non-migratory. Tropical Central America: known only from Isle of Pines, Cuba.
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