Collected from a stream with mixed sand, gravel, pebble, silt, mud and rock bottom, and with clear running water, elevation is 764 m above mean sea level, and with annual rainfall over 1450 mm. Surrounding landscape has semi-evergreen forest, plantations, and agricultural crops (mainly corn, cabbage and tomato). Water temperature was 17°C, air temperature 20.5°C, pH 7.7, DO 6.5 mg/l, and conductivity 20 μS. Averaged stream width 4 meters, and water depth 40-60 cm. Specimens were collected during the daylight by electro-fishing. Other species collected at the type locality were Schistura breviceps, Schistura maejotigrina, Oreoglanis siamensis, Scaphiodonichthys burmanicus, Opsarius pulchellus, Garra cambodgiensis, Puntius brevis, Puntius stoliczkanus, Monopterus albus, Channa gachua and Devario sp. A 3.0 cm SL female (MARNM 3999/14) contained 260 large eggs (0.6 mm diameter) .
This species is distinguished from its congeners by the following set of characters: lateral line incomplete with 62-83 pores, reaching between anal-fin origin and anal-fin tip; absence of axillary lobe on pelvic-fin base; male with hammer head-shaped suborbital flap .Cross section: oval.
Behavioural data for Physoschistura Chulabhornae is currently being reviewed.
Freshwater; demersal; pH range: ? - 7.7; depth range 0 - 1 m . Tropical; ? - 17°C
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