Inhabits coral and rocky reefs. A 5.9 cm SL female (USNM 263738) contains about 60 eggs, 0.4-0.6 mm in diameter and 140 embryos, 4-5 mm TL, with 2 rows of 2-3 large spots along anal and dorsal fin bases .
Dorsal soft rays (total): 63 - 73; Anal soft rays: 47 - 54; Vertebrae: 38 - 41. The species is distinguished by the following: Vertebrae 11-12 + 27-30 = 38-41, dorsal fin rays 63-73, anal fin rays 47-54, outer pseudoclasper very slender, long, wing-shaped, slightly expanded posterodistally, and with single, almost straight supporter; inner pseudoclasper long, stalked, distally with three branches, anterior branch curved, median branch stout, posterior branch fleshy ear-lobed flap; penis tapering; opercular spine with single, sharp tip in small specimens and furcated (2-3 tips) in specimens greater than 45 mm SL; scale patch on cheeks with 7-8 vertical scale rows; otolith length: height ratio 2.4-2.5 .
Behavioural data for Notchspine brotula is currently being reviewed.
Diet information for Notchspine brotula is currently being compiled.
Reproduction data for Notchspine brotula is currently being compiled.
Marine; reef-associated; depth range 0 - 15 m . Tropical; 23°N - 6°N recorded from the region in between.
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