Occurs in a typical annual pool with many aquatic plants of the genera Echinodorus, Utricularia, and Nymphaea. The pool has about 800 m length and 300 m width, 1.60 m maximum depth, substrate consisting of clay, and transparent and slightly dark color water. This was the only fish species encountered at the pool, which was shared with many aquatic insect larvae and several tadpole species .
Dorsal soft rays (total): 16 - 28; Anal soft rays: 16 - 21; Vertebrae: 25. : fusiform / normal.
Behavioural data for Hypsolebias Trifasciatus is currently being reviewed.
Diet information for Hypsolebias Trifasciatus is currently being compiled.
Reproduction data for Hypsolebias Trifasciatus is currently being compiled.
Freshwater; benthopelagic. Tropical South America: Rio Preto, rio São Francisco basin in Brazil.
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