This species was collected exclusively in solid ground forest streams, both the streams tributaries of Río Morona (e.g., quebrada Uchpayacu, quebrada Katmaentsa, and quebrada Monshurbac), as well as the quebrada Platanoyacu (tributary of Río Corrientes) and streams near Leticia where the species was recorded are blackwater streams. For a stream near Leticia from where the species was found and observed, the Caño Pichuna has pH 6.83, conductivity 18 μS/cm, and temperatrure (at 12:50 p.m.) of 26.5°C. Recorded food items include larvae of aquatic Diptera, Hymenoptera (presumably ants), Homoptera, Ephemeroptera, aquatic Coleoptera, and larvae of terrestrial Diptera (Phoridae). Reported fecundity of 136 oocytes for mature females were collected in November .
Dorsal spines (total): 2; Dorsal soft rays (total): 8 - 9; Anal spines: 4; Anal soft rays: 20 - 23. This species of the Holopristis ocellifer species-group (sensu Lima & Sousa, 2009) is distinguished by the following characters: two humeral blotches and a caudal peduncle blotch. In that group: the second humeral blotch larger and considerably darker than the first humeral blotch (vs. first humeral blotch larger and considerably more conspicuous than the second humeral blotch, which is vertically elongated and narrow), except H. yinyang to which it differs by the presence of a caudal peduncle blotch (vs. caudal peduncle blotch absent).Cross section: oval.
Behavioural data for Holopristis Aguaruna is currently being reviewed.
Diet information for Holopristis Aguaruna is currently being compiled.
Reproduction data for Holopristis Aguaruna is currently being compiled.
Freshwater; benthopelagic. Tropical South America: Peru, Colombia, possibly Brazil and Ecuador.
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