Subtidal species found in rocky areas among benthic encrusting communities in algal cover or kelp holdfasts . Minimum depth from Ref. 58018.
Dorsal spines (total): 38 - 43; Dorsal soft rays (total): 14 - 18; Anal spines: 2; Anal soft rays: 42 - 44; Vertebrae: 60 - 62. Pelvic fins present, each of a minute spine; first dorsal spine associated with first pterygiophore; first anal pterygiophore noticeably more massive than second; ventrolateral lateral line not connected; meridioventral lateral line absent . Caudal fin rounded.
Behavioural data for Twoline prickleback is currently being reviewed.
Marine; demersal; depth range 0 - 46 m . Subtropical Central Pacific: Farallon Islands, California to Punta Banda, Baja California Norte, Mexico.
Occurrence records from the Global Biodiversity Information Facility (GBIF) - 3 records indexed .
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